Plasmid

Part:BBa_J23001:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-06-20)


pSB3C6-J01022 p15A+CmR plasmid with OnRFP part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1083
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1945
    Illegal SpeI site found at 1084
    Illegal PstI site found at 1098
    Illegal NotI site found at 7
    Illegal NotI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1084
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1084
    Illegal PstI site found at 1098
    Illegal AgeI site found at 794
    Illegal AgeI site found at 906
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

p15A plasmids are low/medium copy (~15 /cell). Yield and quality of DNA preps may be low.


Source

pACYC184 (in many steps)

References